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       Capas de Revistas


       Polymers 9(11) (2017)


Tânia Ribeiro, Ana Margarida Santiago, Jose Manuel Gaspar Martinho and Jose Paulo Farinha

A Cationic Smart Copolymer for DNA Binding

A new copolymer with a temperature-responsive block and a cationic block was prepared by reversible addition–fragmentation chain transfer (RAFT) polymerization. The temperature-responsive block of di(ethylene glycol) methyl ether methacrylate (DEGMA) and oligo(ethylene glycol) methyl ether methacrylate (OEGMA) has tunable volume phase transition temperature depending on the DEGMA/OEGMA ratio. The cationic block of trimethyl-2-methacroyloxyethylammonium chloride (TMEC) is charged at physiological pH values and was used for binding single strand DNA. The copolymer forms coacervate complexes with the DNA, which were characterized by fluorescence correlation spectroscopy (FCS) and fluorescence spectroscopy. The new materials offer good prospects for application in controlled gene delivery.


       Phys Chem Chem Phys. 16 (2017) (Outside Cover)


Inês F. A. Mariz, Sandra Pinto, João Lavrado, Alexandra Paulo, José M. G. Martinho, and Ermelinda M. S. Maçôas

Cryptolepine and quindoline: understanding their photophysics.

The insight provided this study on the fundamental photophysical properties of cryptolepine and quindoline, the building blocks of many anti-malaria agents based on indoloquinolines, can be used to anticipate the light emitting properties of the corresponding derivatives, prepared by functionalization of the fused ring core, in different environments within the parasite intraerythrocytic stage (inside the nucleus, in lysosomes or in the digestive vacuole).



       Chem. Eng. J. 319 (2017)


S. P. C. Alves, C. Santos, A. P. da Costa, M. Silva, C. Baleizão, J. P. S. Farinha, "Smart polymeric nanoparticles for boron scavenging"



Novel boron scavenging process. At room temperature the nanoparticles are expanded, promoting boron chelation to diol groups. At higher temperature the polymer shell collapses inducing nanoparticle flocculation. The nanoparticles can then be regenerated by pH treatment and reused.


T. Sousa, R.E. Castro, S.N. Pinto, A. Coutinho, S.D. Lucas, R. Moreira, C.M.P. Rodrigues, M. Prieto, F. Fernandes, “Deoxycholic acid modulates cell death signaling through changes in mitochondrial membrane properties.”


A imagem mostra resultados de imagiologia raciométrica espectral de fluorescência e de tempos de vida de fluorescência (FLIM) de sondas sensíveis à ordem de membranas em células HEK293T, após tratamento com concentrações fisiologicamente ativas de ácidos biliares apoptóticos (DCA) e citoprotectores (UDCA e TUDCA).


       Chem. Phys, 449, 23-33 (2015)


M.N. Berberan-Santos, «Phasor plots of luminescence decay functions».

A imagem mostra a secção do espaço "phasor" com curvas características correspondendo a vários tipos de leis de decaimento (sub-exponencial, exponencial, super-exponencial).



       ChemCatChem, 2, 254-260 (2015) (Capa, interior)


R. Ciriminna, A. Fidalgo, V. Pandarus, F. Béland, L.M. Ilharco, M. Pagliaro, «New Catalyst Series from the Sol–Gel-Entrapment of Gold Nanoparticles in Organically Modified Silica Matrices: Proof of Performance in a Model Oxidation Reaction».

A figura mostra a imagem TEM de novas nanopartículas confinadas na matriz ORMOSIL de catalisador SiliaCat Au como fundo, com o esquema reaccional, para a oxidação modelo do 1-feniletanol com peróxido de hidrogénio sobreposto.



       Phys. Chem. Chem. Phys. 16 (34) (2014) (Capa, interior)


A. Melo, A. Fedorov, M. Prieto, A. Coutinho. "Exploring homo-FRET to quantify the oligomer stoichiometry of membrane-bound proteins involved in a cooperative partition equilibrium."

A figura mostra o processo de migração de energia após excitação de um agregado proteico hexamérico, induzido pela interação proteína-lípido.



        Soft Matter, 10(6) (2014) (Contracapa)


A.M. Melo, L.M.S. Loura, F. FErnandes, J. Villalaín, M. Prieto, A. Coutinho, "Electrostatically driven lipid–lysozyme mixed fibers display a multilamellar structure without amyloid features".

Reproduction of lipid-protein fiber structure. The lipid-protein mixed fibers present a multilayer structure, in which the predominantly oligomeric lysozyme is sandwiched between two adjacent lipid bilayers.



        Polymer Chemistry, 4 (2013)


Relogio,P.; Bathfield, M.; Haftek-Terreau, Z.; Beija, M.; D’Agosto, F.; Favier, A.; Giraud-Panis, M.-J.; Mandrand, B.; Farinha, J. P. S.; Charreyre, M.-T.; Martinho, J. M. G., "Biotin-End-Functionalized Highly Fluorescent Water-Soluble Polymers".

The cover image is a reproduction of the painting "Lucifer Yellow" by Ana Tristany , of four polymer chains (blue) labeled with several LY fluorophores (yellow), each with a biotin at one chain-end (blue/black). The biotin groups are in the process of interacting with a streptavidine protein (red).



        The Journal of Physical Chemistry B, 117 (2013)


A. Melo, J. Ricardo, A. Fedorov, M. Prieto, A. Coutinho. "Fluorescence Detection of Lipid-Induced Oligomeric Intermediates Involved in Lysozyme “Amyloid-Like” Fiber Formation Driven by Anionic Membranes."

Lipid–Protein Fibers Formed upon Lysozyme Interaction with Negatively Charged Membranes.



        The Journal of Lipid Research, 53(3) (2012)


L. C. Silva, O. B.David, Y. Pewzner-Jung, E. L. Laviad, J. Stiban, S. Bandyopadhyay, A. H. Merrill Jr., M. Prieto and A.H. Futerman.. "Fluorescence Detection of Lipid-Induced Oligomeric Intermediates Involved in Lysozyme “Ablation of ceramide synthase 2 strongly affects biophysical properties of membranes."

Effect of CerS2 ablation on biophysical properties of liver membranes.



European Biophysical Journal, capa de todos os exemplares de 2012, utilizando figuras de artigos seleccionados de anos anteriores.


L.M.S. Loura, F. Fernandes, M. Prieto, "Quantification of protein–lipid selectivity using FRET". Eur. Biophys. J. 39, 2010.



        ChemPhysChem - A European Journal of Chemical Physics and Physical Chemistry, 12 (2011)


C. Baleizão, M.N. Berberan-Santos, "The Brightest Fullerene. A New Isotope Effect in Molecular Fluorescence and Phosphorescence".



        Journal of Neurochemistry, 116(5) (2011)


A. Coutinho, L.M.S. Loura, M. Prieto, "FRET studies of lipid-protein aggregates related to amyloid-like fibers"


        The Journal of Physical Chemistry C, 112 (2008)


J.P.S. Farinha, J.M.G. Martinho, "Resonance Energy Transfer Between Dyes in Nanosized Domains at Polymer Interfaces".

Resonance energy transfer in nano-size domains at the interface between different polymers: The cover is a reproduction of the painting "Interfaces" (acrylic on canvas, 1.5 m x 1.5 m), a personal interpretation by artist Ana Tristany of nano-size domains located at the interface between different polymers (in black and grey), labeled with energy donor (red) and acceptor (green) dyes. The plot in white was pasted onto the image and represents the concentration profile of the dyes at the interfacial domain. The nanometer size dimensions of the domains match the typical lengthscale of FRET, allowing the determination of the size and distribution functions. The review paper Energy Transfer in Polymer Nanodomains, describe the tools necessary to extract information on the dyes distribution and the morphology of the interfacial domains



        Biophysical Journal, 95 (2008)


S.N. Pinto, L. C. Silva, R. F. M. de Almeida, M. Prieto, "Membrane domain formation, interdigitation and morphological alterations induced by the very long chain asymmetric C24:1 ceramide".



actualização: 15.03.2018